Electrochemical detection of a breast cancer susceptible gene using cDNA immobilized chitosan-co-polyaniline electrode |
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| Authors: | Ashutosh Tiwari Shaoqin Gong |
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| Affiliation: | a Department of Mechanical Engineering, University of Wisconsin-Milwaukee, 3200 North Cramer Street, Milwaukee, WI 532011, USA
b Department of Materials, University of Wisconsin-Milwaukee, 3200 North Cramer Street, Milwaukee, WI 532011, USA |
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| Abstract: | An electrochemical breast cancer biosensor based on a chitosan-co-polyaniline (CHIT-co-PANI) copolymer coated onto indium-tin-oxide (ITO) was fabricated by immobilizing the complementary DNA (cDNA) probe (42 bases long) associated with the breast cancer susceptible gene BRCA1. Both the CHIT-co-PANI/ITO and the cDNA/CHIT-co-PANI/ITO electrodes were characterized with Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). For the cDNA/CHIT-co-PANI/ITO electrode, the amperometric current decreased linearly with an increasing logarithm of molar concentration of the single-stranded target DNA (ssDNA) within the range of 0.05-25 fmol. The bioelectrode exhibited a sensitivity of 2.104 μA/fmol with a response time of 16 s. The cDNA/CHIT-co-PANI/ITO electrode had a shelf life of about six months, even when stored at room temperature. |
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| Keywords: | Chitosan-co-polyaniline Breast cancer detection cDNA immobilization Electrochemical biosensor |
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