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Effect of a carboxyl group on the chemiluminescent reaction of tris(2,2′-bipyridine)ruthenium(III) with aliphatic amines |
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| Authors: | Hitoshi Kodamatani Shigeo Yamazaki |
| Affiliation: | a Department of Earth and Environmental Sciences, Faculty of Science, Kagoshima University, 1-21-35 Korimoto, Kagoshima 890-0065, Japan b Environmental Research Institute, Kanazawa Institute of Technology, 3-1 Yatsukaho, Hakusan 924-0838, Japan c Department of Applied Science, Okayama University of Science, 1-1 Ridaicho, Okayama 700-0005, Japan d Division of Human Environment, Graduate School of Human Development and Environment, Kobe University, 3-11 Tsurukabuto, Nada, Kobe 657-8501, Japan |
| Abstract: | The effect of a carboxyl group beside nitrogen of aliphatic amines on the tris(2,2′-bipyridine)ruthenium(III), Ru(bpy)33+, chemiluminescent reaction was examined. It has been shown that a carboxylate anion promotes the chemiluminescent reaction at a lower pH and then the aliphatic amines with this substituent can be sensitively detected compared with corresponding aliphatic amines without this substituent. Based on this finding, preliminary studies on simultaneous determination of 4-hydroxyproline, N-methylglycine, N-methylalanine, proline, and pipecolic acid in human serum have been performed using isocratic reversed-phase ion-pair high-performance liquid chromatography (HPLC) with electrogenerated Ru(bpy)33+ chemiluminescent detection. The detection limits (signal-to-noise ratio of 3) with the proposed method were 3.0, 12, 2.7, 4.6, and 10 nM for 4-hydroxyproline, N-methylglycine, N-methylalanine, proline, and pipecolic acid, respectively. |
| Keywords: | Aliphatic amine Amino acid Carboxyl group Tris(2,2&prime -bipyridine)ruthenium(III) Chemiluminescence |
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