| Microcalorimetric Evaluation of the Effect of Kanamycin： An Analysis Based on the Median-Effect Principle |
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| 引用本文： | 王莉衡,范代娣,尚龙安,施惠娟,马晓轩,米钰,顾利锋,徐抗震.Microcalorimetric Evaluation of the Effect of Kanamycin： An Analysis Based on the Median-Effect Principle[J].中国化学,2007,25(1):20-24. |
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| 作者姓名： | 王莉衡 范代娣 尚龙安 施惠娟 马晓轩 米钰 顾利锋 徐抗震 |
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| 作者单位： | [1]Department of Chemical Engineering, Northwest University/Shaanxi Source and Application Chemical Engineering Center, Xi＇an, Shaanxi 710069, China [2]Key Laboratory of Resource Biology and Biotechnology in Western China （Northwest University）, Ministry of Education, Xi＇an, Shaanxi 710069, China [3]Collage of Biological and Chemical Engineering Ningbo Institute of Technology, Zhejiang Univerisity, Ningbo, Zhejiang 315100, China [4]Department of Reproductive Biology, Shanghai Institute of Planned Parenthood Research,Shanghai 200032, China |
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| 基金项目： | Project supported by the National Science and Technology Key Funds （No. 2003DA901 A32） and the National Natural Science Foundation of China （No. 20476085）. |
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| 摘 要： | A study of the effect of drug, kanamycin, on the growth metabolism of recombinant Escherichia coli B 1 was carded out by microcalorimeter monitoring of the metabolic activity of treated cells. Power-time curves of growing recombinant Escherichia coli cell suspensions, treated with different kanamycin doses, were recorded. The extent of the effect was evaluated by changes in the slopes of the microcalorimetric curves and the kinetics of the drug action was interpreted from the time at which these changes reached their maximum values and maintained their maximum values. Experimental dose-effect relationships conform to the median-effect principle of the mass-action law： fa/（1-fa）=（D/D50）^m. A plot of y=lg（fa）^1-1]^-1 versus x=lg D gives the slope m, D50 and R∞. The experimental results revealed that high concentration of kanamycin had an inhibitory effect on the growth of recombinant Escherichia coli B 1 in the lg phase, and had a promoting effect in the stationary period. Moreover, it was demonstrated that microcalorimetry was a reliable method for the detection of modulatory effects in biology.
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| 关 键 词： | 卡那霉素 微量热法 药效评价 重组大肠埃希氏菌 中效原理 剂量效应 |
| 修稿时间： | 2006-04-242006-09-21 |
Microcalorimetric Evaluation of the Effect of Kanamycin: An Analysis Based on the Median‐Effect Principle |
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| Li‐Heng Wang,Dai‐Di Fan,Long‐An Shang,Hui‐Juan Shi,Xiao‐Xuan Ma,Yu Mi,Li‐Feng Gu,Kang‐Zhen Xu.Microcalorimetric Evaluation of the Effect of Kanamycin: An Analysis Based on the Median‐Effect Principle[J].Chinese Journal of Chemistry,2007,25(1):20-24. |
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| Authors: | Li‐Heng Wang Dai‐Di Fan Long‐An Shang Hui‐Juan Shi Xiao‐Xuan Ma Yu Mi Li‐Feng Gu Kang‐Zhen Xu |
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| Institution: | 1. Department of Chemical Engineering, Northwest University/Shaanxi Source and Application Chemical Engineering Center, Xi'an, Shaanxi 710069, China;2. Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an, Shaanxi 710069, China;3. Collage of Biological and Chemical Engineering Ningbo Institute of Technology, Zhejiang Univerisity, Ningbo, Zhejiang 315100, China;4. Department of Reproductive Biology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China |
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| Abstract: | A study of the effect of drug, kanamycin, on the growth metabolism of recombinant Escherichia coli B1 was carried out by microcalorimeter monitoring of the metabolic activity of treated cells. Power‐time curves of growing recombinant Escherichia coli cell suspensions, treated with different kanamycin doses, were recorded. The extent of the effect was evaluated by changes in the slopes of the microcalorimetric curves and the kinetics of the drug action was interpreted from the time at which these changes reached their maximum values and maintained their maximum values. Experimental dose‐effect relationships conform to the median‐effect principle of the mass‐action law: fa/(1?fa)=(D/D50)m. A plot of y=lg(fa)1?1]?1 versus x=lg D gives the slope m, D50 and R∞. The experimental results revealed that high concentration of kanamycin had an inhibitory effect on the growth of recombinant Escherichia coli B1 in the lg phase, and had a promoting effect in the stationary period. Moreover, it was demonstrated that microcalorimetry was a reliable method for the detection of modulatory effects in biology. |
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| Keywords: | kanamycin microcalorimetry recombinant Escherichia coli |
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