| Interaction of Co（ll） with Bovine Serum Albumin under UV C Irradiation |
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| 引用本文： | 边贺东 张海蓉 于青 梁宏 倪寿海. Interaction of Co（ll） with Bovine Serum Albumin under UV C Irradiation[J]. 中国化学, 2007, 25(8): 1116-1120. DOI: 10.1002/cjoc.200790208 |
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| 作者姓名： | 边贺东 张海蓉 于青 梁宏 倪寿海 |
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| 作者单位： | College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China （No. 20671023）, the Science Foundation of Guangxi Province, China, and the Teaching and Research Award Programme for 0utstanding Young Teachers in Higher Education Institutions of Ministry of Education, China. |
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| 摘 要： | The interaction of Co（Ⅱ） with BSA under UV C （253.7 nm） irradiation under physiological conditions has been studied by UV-vis spectrum, ultraviolet second-derivative spectroscopy and fluorescence spectrum. The quenching rate constant kq and the association constant Ka were calculated according to Stern-Volmer equation based on the quenching of the fluorescence of BSA by Co（Ⅱ）.
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| 关 键 词： | 钴(Ⅱ) BSA 粘接 荧光淬火 紫外光谱 紫外照射 |
| 修稿时间： | 2007-01-212007-04-26 |
Interaction of Co（ll） with Bovine Serum Albumin under UV C Irradiation |
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| BIAN He-Dong, ZHANG Hai-Rong, YU Qing,LIANG Hong, NI Shou-Hai. Interaction of Co（ll） with Bovine Serum Albumin under UV C Irradiation[J]. Chinese Journal of Chemistry, 2007, 25(8): 1116-1120. DOI: 10.1002/cjoc.200790208 |
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| Authors: | BIAN He-Dong ZHANG Hai-Rong YU Qing LIANG Hong NI Shou-Hai |
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| Affiliation: | College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China |
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| Abstract: | The interaction of Co(II) with BSA under UV C (253.7 nm) irradiation under physiological conditions has been studied by UV‐vis spectrum, ultraviolet second‐derivative spectroscopy and fluorescence spectrum. The quenching rate constant kq and the association constant Ka were calculated according to Stern‐Volmer equation based on the quenching of the fluorescence of BSA by Co(II). |
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| Keywords: | Co（Ⅱ） BSA binding fluorescence quenching UV-vis spectra ultraviolet second-derivative spectroscopy UV C irradiation |
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