| Studies on the Interaction of Dinitratobis（phen） Cadmium Complex with DNA |
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| 引用本文： | 袁彩霞 吴艳波 魏毅斌 杨频 朱苗力. Studies on the Interaction of Dinitratobis（phen） Cadmium Complex with DNA[J]. 中国化学, 2007, 25(9): 1267-1272. DOI: 10.1002/cjoc.200790235 |
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| 作者姓名： | 袁彩霞 吴艳波 魏毅斌 杨频 朱苗力 |
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| 作者单位： | Institute of Molecular Science, the Key Laboratory of Chemical Biology and Molecular Engineering of Education Ministry, Shanxi University, Taiyuan, Shanxi 030006, China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China （Nos. 20601018, 20471033） and the Natural Science Foundation of Shanxi Province （No. 20051013）.Acknowledgement The authors are grateful to Prof. Si-Dian Li （Materials Sciences and Department of Chemistry, Xinzhou Teachers＇ University） for the support of computational resources. |
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| 摘 要： | DNA-binding properties of the dinitratobis（phen） cadmium complex [Cd（phen）2（NO3）2] （where phen = 1,10-phenanthroline） have been investigated with absorption titration, fluorescence spectroscopy, viscosity measurement, molecular modeling and density functional theory （DFT） calculations. The results indictate DNA-binding mode of the complex to be weak groove binding rather than partial intercalative interaction expected of the extended planar aromatic phen ring. In addition, the DNA cleavage study was carried out by gel electrophoresis experiment. The results showed that the complex both hardly cleaves pBR322 DNA in the absence and present ascorbate. So it is suggested that the formation of cadmium complex can decrease cadmium toxicity to some extents.
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| 关 键 词： | 镉元素 复合物 密度函数理论 DNA交互作用 |
| 修稿时间： | 2007-01-04 |
Studies on the Interaction of Dinitratobis（phen） Cadmium Complex with DNA |
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| YUAN Cai-Xia, WU Yan-Bo, WEI Yi-Bin, YANG Pin, ZHU Miao-Li. Studies on the Interaction of Dinitratobis（phen） Cadmium Complex with DNA[J]. Chinese Journal of Chemistry, 2007, 25(9): 1267-1272. DOI: 10.1002/cjoc.200790235 |
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| Authors: | YUAN Cai-Xia WU Yan-Bo WEI Yi-Bin YANG Pin ZHU Miao-Li |
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| Affiliation: | Institute of Molecular Science, the Key Laboratory of Chemical Biology and Molecular Engineering of Education Ministry, Shanxi University, Taiyuan, Shanxi 030006, China |
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| Abstract: | DNA‐binding properties of the dinitratobis(phen) cadmium complex [Cd(phen)2(NO3)2] (where phen?1,10‐phenanthroline) have been investigated with absorption titration, fluorescence spectroscopy, viscosity measurement, molecular modeling and density functional theory (DFT) calculations. The results indictate DNA‐binding mode of the complex to be weak groove binding rather than partial intercalative interaction expected of the extended planar aromatic phen ring. In addition, the DNA cleavage study was carried out by gel electrophoresis experiment. The results showed that the complex both hardly cleaves pBR322 DNA in the absence and present ascorbate. So it is suggested that the formation of cadmium complex can decrease cadmium toxicity to some extents. |
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| Keywords: | cadmium complex DNA interaction density functional theory （DFT） |
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