| Microcalorimetric Studies on Gene Promoter Function of Cloned DNA Fragements from Halobacterium halobium J7 Plasmid pHH205 in Escherichia coli TG1 |
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| 引用本文： | 雷克林 侯汉娜 刘义 叶学成 沈萍. Microcalorimetric Studies on Gene Promoter Function of Cloned DNA Fragements from Halobacterium halobium J7 Plasmid pHH205 in Escherichia coli TG1[J]. 中国化学, 2007, 25(8): 1099-1104. DOI: 10.1002/cjoc.200790205 |
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| 作者姓名： | 雷克林 侯汉娜 刘义 叶学成 沈萍 |
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| 作者单位： | [1]College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei 430072, China [2]Department of Chemistry, Xiangfan University, Xiangfan, Hubei 441003, China [3]State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China （Nos. 30570015, 20373051）, ＂973＂ Project （No. 2004CB719603）, Science Fund for Creative Research Group （No. 20621502 NSFC）, Science Research Foundation of Ministry of Education of China （No. [2006]8IRT0543）, and the Natural Science Foundation of Hubei Province （No. 2005ABC02）. |
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| 摘 要： | Halobacterium halobium is a typical kind of extremely halophilic bacterium. Combined with the antibiotic resistance assay, the microcalorimetric method was used to study the promoter function of the cloned DNA fragments from Halobacterium halobium J7 plasmid pHH205 in Escherichia coli TG1. The promoter probe vector, plasmid pKK232-8, was used to form the recombinants. The DNA fragment, which is the promoter for the chloramphenicol acetyl transferase （CAT） gene in plasmid pKK232-8, is about 800 bp, and the chloramphenicol resistance level presented by IC50 is about 200 μg·mL^-1, which suggests a high promoter activity. The conclusions show that there probably exist double-function or trinary-function gene promoters in Halobacterium halobium, and Archaea may contain rich genetic resources.
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| 关 键 词： | 微量热法 基因催化 DNA 埃希氏菌属 |
| 修稿时间： | 2006-06-292007-05-16 |
Microcalorimetric Studies on Gene Promoter Function of Cloned DNA Fragements from Halobacterium halobium J7 Plasmid pHH205 in Escherichia coli TG1 |
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| LEI Ke-Lin,HOU Han-Na, LIU Yi, YE Xue-Cheng, SHEN Ping. Microcalorimetric Studies on Gene Promoter Function of Cloned DNA Fragements from Halobacterium halobium J7 Plasmid pHH205 in Escherichia coli TG1[J]. Chinese Journal of Chemistry, 2007, 25(8): 1099-1104. DOI: 10.1002/cjoc.200790205 |
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| Authors: | LEI Ke-Lin HOU Han-Na LIU Yi YE Xue-Cheng SHEN Ping |
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| Affiliation: | 1.College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei 430072, China ;2. Department of Chemistry, Xiangfan University, Xiangfan, Hubei 441003, China ;3. State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, China |
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| Abstract: | Halobacterium halobium is a typical kind of extremely halophilic bacterium. Combined with the antibiotic resistance assay, the microcalorimetric method was used to study the promoter function of the cloned DNA fragments from Halobacterium halobium J7 plasmid pHH205 in Escherichia coli TG1. The promoter probe vector, plasmid pKK232‐8, was used to form the recombinants. The DNA fragment, which is the promoter for the chloramphenicol acetyl transferase (CAT) gene in plasmid pKK232‐8, is about 800 bp, and the chloramphenicol resistance level presented by IC50 is about 200 µg·mL?1, which suggests a high promoter activity. The conclusions show that there probably exist double‐function or trinary‐function gene promoters in Halobacterium halobium, and Archaea may contain rich genetic resources. |
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| Keywords: | Halobacterium halobium J7 plasmid pKK232-8 gene promoter microcalorimetry |
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