| Preparation of Medium Cation Exchange Stationary Phase of Polymeric Matrix and Their Chromatographic Properties |
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| 作者姓名： | 陈刚 龚波林 白泉 耿信笃 |
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| 作者单位： | Institute of Modern Separation Science, Key Laboratory of Modern Separation Science in Shaanxi Province, Northwest University, Xi＇an, Shaanxi 710069, China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China （Nos. 39880003, 20175016）. |
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| 摘 要： | Based on the monodisperse poly（glycidyl methacrylate-co-ethylenedimethacrylate） beads （PGMA/EDMA） with macropore as a medium, a new hydrophilic medium cation exchange （MCX） stationary phase for HPLC was synthesized by a new chemically modified method. The stationary phase was evaluated with the property of ion exchange, separability, reproducibility, hydrophilicity, effect of salt concentration, salt types, column loading and pH on the separation and retention of proteins in detail. It was found that it follows ion exchange chromatographic （IEC） retention mechanism. The measured bioactivity recovery for lysozyme was （96 ± 5）%. The dynamic protein loading capacity of the synthesized MCX packings was 21.8 mg/g. Five proteins were almost completely separated within 6.0 min at a flow rate of 4 mL/min using the synthesized MCX resin. The MCX resin was also used for the rapid separation and purification of lysozyme from egg white with only one step. The purity and specific bioactivity of the purified lysozyme was found more than 95% and 70345 U/mg, respectively.
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| 关 键 词： | 介质阳离子交换色谱 固定相 聚合物模板 色谱性质 离子交换色谱 树脂 |
| 修稿时间： | 2006-02-062006-09-01 |
Preparation of Medium Cation Exchange Stationary Phase of Polymeric Matrix and Their Chromatographic Properties |
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| CHEN, Gang, GONG, Bo-Lin, BAI, Quan, GENG, Xin-Du.Preparation of Medium Cation Exchange Stationary Phase of Polymeric Matrix and Their Chromatographic Properties[J].Chinese Journal of Chemistry,2007,25(1):77-82. |
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| Authors: | CHEN Gang GONG Bo-Lin BAI Quan GENG Xin-Du |
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| Institution: | 1. Institute of Modern Separation Science, Key Laboratory of Modern Separation Science in Shaanxi Province, Northwest University, Xi'an, Shaanxi 710069, China;2. Tel.: +0086‐029‐88302808;3. Fax: +0086‐029‐88303817 |
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| Abstract: | Based on the monodisperse poly(glycidyl methacrylate‐co‐ethylenedimethacrylate) beads (PGMA/EDMA) with macropore as a medium, a new hydrophilic medium cation exchange (MCX) stationary phase for HPLC was synthesized by a new chemically modified method. The stationary phase was evaluated with the property of ion exchange, separability, reproducibility, hydrophilicity, effect of salt concentration, salt types, column loading and pH on the separation and retention of proteins in detail. It was found that it follows ion exchange chromatographic (IEC) retention mechanism. The measured bioactivity recovery for lysozyme was (96±5)%. The dynamic protein loading capacity of the synthesized MCX packings was 21.8 mg/g. Five proteins were almost completely separated within 6.0 min at a flow rate of 4 mL/min using the synthesized MCX resin. The MCX resin was also used for the rapid separation and purification of lysozyme from egg white with only one step. The purity and specific bioactivity of the purified lysozyme was found more than 95% and 70345 U/mg, respectively. |
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| Keywords: | monodisperse poly(glycidyl methacrylate‐co‐ethylenedimethacrylate) resin medium cation exchange chromatography protein separation egg white lysozyme |
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