| Investigation of the Interaction between Isoflavonoids and Bovine Serum Albumin by Fluorescence Spectroscopy |
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| 引用本文： | 屈凌波 陈晓岚 杨冉 王玲 曾华金. Investigation of the Interaction between Isoflavonoids and Bovine Serum Albumin by Fluorescence Spectroscopy[J]. 中国化学, 2007, 25(8): 1151-1155. DOI: 10.1002/cjoc.200790215 |
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| 作者姓名： | 屈凌波 陈晓岚 杨冉 王玲 曾华金 |
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| 作者单位： | [1]Department of Chemistry, Anyang Normal University, Anyang, Henan 455002, China [2]Key Laboratory of Chemistry Biology, Department of Chemistry, Zhengzhou University, Zhengzhou, Henan 450052, China [3]College of Pharmacy, Zhengzhou University, Zhengzhou, Henan 450001, China |
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| 基金项目： | Project supported by the Natural National Science Foundation （Nos. 20132020 and 20175026） of China and the Henan Academic Foundation of Science and Technology. |
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| 摘 要： | The interactions of bovine serum albumin （BSA） with three structurally related isoflavonoids, genistein, puerarin and daidzein, were studied under physiological conditions by fluorescence spectroscopic technique. The quenching mechanism of these compounds with BSA was suggested as static quenching and the binding constants were determined at different temperatures based on the fluorescence quenching results. The transfer efficiency of energy and distance between the acceptor and BSA were investigated on the basis of the mechanism of the Forster energy transference. According to the thermodynamic parameters it has been suggested that the acting force be mainly hydrophobic force. The comparison of binding potency of the three isoflavonoids to BSA showed that the substitution by 5-OH and 8-Glc could enhance the binding affinity. All these obtained in the work can make us better understand the mode of the action and pharmacological activities of the isoflavonoids.
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| 关 键 词： | 牛血清白蛋白 异类黄酮 染料木碱 葛根素 大豆黄酮 荧光光谱 |
| 修稿时间： | 2006-08-212007-04-22 |
Investigation of the Interaction between Isoflavonoids and Bovine Serum Albumin by Fluorescence Spectroscopy |
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| QU Ling-Bo, CHEN Xiao-Lan,YANG Ran, WANG Ling,ZENG Hua-Jin. Investigation of the Interaction between Isoflavonoids and Bovine Serum Albumin by Fluorescence Spectroscopy[J]. Chinese Journal of Chemistry, 2007, 25(8): 1151-1155. DOI: 10.1002/cjoc.200790215 |
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| Authors: | QU Ling-Bo CHEN Xiao-Lan YANG Ran WANG Ling ZENG Hua-Jin |
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| Affiliation: | 1.Department of Chemistry, Anyang Normal University, Anyang, Henan 455002, China ;2. Key Laboratory of Chemistry Biology, Department of Chemistry, Zhengzhou University, Zhengzhou, Henan 450052, China ;3. College of Pharmacy, Zhengzhou University, Zhengzhou, Henan 450001, China |
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| Abstract: | bovine serum albumin, isoflavonoid, genistein, puerarin, daidzein, fluorescence spectroscopy, fluorescence quenching |
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| Keywords: | bovine serum albumin isoflavonoid genistein puerarin daidzein fluorescence spectroscopy fluorescence quenching |
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