| Poly（dimethylsiloxane） Microchips with Two Sharpened Stretching Tips and Its Application to Protein Separation Using Dynamic Coating |
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| 引用本文： | 刘春叶,许旭,高红军,陈杰瑢.Poly（dimethylsiloxane） Microchips with Two Sharpened Stretching Tips and Its Application to Protein Separation Using Dynamic Coating[J].中国化学,2007,25(2):190-195. |
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| 作者姓名： | 刘春叶 许旭 高红军 陈杰瑢 |
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| 作者单位： | [1]School of Life Science and Technology, Xi＇an Jiaotong University, Xi＇an, Shaanxi 710049, China [2]Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China |
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| 摘 要： | An integrated poly（dirnethylsiloxane） （PDMS） microchip with two sharpened stretching tips for convenient sample injecting, running buffer refreshing and channel cleaning has been presented. The sample was directly introduced into the separation channel through the stretching inlet tip without complicated power switching supplies and injection cross channel. The operation of running buffer refreshing or channel cleaning was simplified by vacuuming one end of the tip and placing the other tip into the solution vial. Therefore, this fabrication method can be easily applied to most analytical laboratories economically without soft lithography and plasma bonding equipments. The attractive performance of the novel PDMS microchips has been demonstrated by using laser-induced fluorescence detection for separation of proteins. The addition of 0.04% Brij 35 in 0.04 mol/L phosphate buffer （pH 7.0） can reduce the adhesion of proteins in multienzyme tablet and make separation more easily. The electroosmotic flow （EOF） exhibits pH-independence in the range of 3-1 1 in dynamic modified microchannel.
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| 关 键 词： | 微芯片 硅 集成电路 制作成本 |
| 修稿时间： | 2006-08-282006-11-06 |
Poly(dimethylsiloxane) Microchips with Two Sharpened Stretching Tips and Its Application to Protein Separation Using Dynamic Coating |
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| LIU, Chun-Ye XU, Xu GAO, Hong-Jun CHEN, Jie-Rong.Poly(dimethylsiloxane) Microchips with Two Sharpened Stretching Tips and Its Application to Protein Separation Using Dynamic Coating[J].Chinese Journal of Chemistry,2007,25(2):190-195. |
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| Authors: | LIU Chun-Ye XU Xu GAO Hong-Jun CHEN Jie-Rong |
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| Institution: | 1. School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China;2. Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China;3. 0086‐021‐54925320 |
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| Abstract: | An integrated poly(dimethylsiloxane) (PDMS) microchip with two sharpened stretching tips for convenient sample injecting, running buffer refreshing and channel cleaning has been presented. The sample was directly introduced into the separation channel through the stretching inlet tip without complicated power switching supplies and injection cross channel. The operation of running buffer refreshing or channel cleaning was simplified by vacuuming one end of the tip and placing the other tip into the solution vial. Therefore, this fabrication method can be easily applied to most analytical laboratories economically without soft lithography and plasma bonding equipments. The attractive performance of the novel PDMS microchips has been demonstrated by using laser‐induced fluorescence detection for separation of proteins. The addition of 0.04% Brij 35 in 0.04 mol/L phosphate buffer (pH 7.0) can reduce the adhesion of proteins in multienzyme tablet and make separation more easily. The electroosmotic flow (EOF) exhibits pH‐independence in the range of 3–11 in dynamic modified microchannel. |
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| Keywords: | poly(dimethylsiloxane) microfluidic chip sampling dynamic coating protein |
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