| DNA Cleavage Promoted by Cu^2＋ Complex of N,N＇-Bis（2-aminoethyl）-2,6-pyridinedicarboxamide |
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| 引用本文： | 李英,盛欣,邵莺,陆国元.DNA Cleavage Promoted by Cu^2＋ Complex of N,N＇-Bis（2-aminoethyl）-2,6-pyridinedicarboxamide[J].中国化学,2007,25(7):937-941. |
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| 作者姓名： | 李英 盛欣 邵莺 陆国元 |
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| 作者单位： | Department of Chemistry, State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing, Jiangsu 210093, China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China （No. 20372032） and the Analytical Foundation of Nanjing University. |
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| 摘 要： | The interaction of Cu^2＋ complex of N,N＇-bis（2-aminoethyl）-2,6-pyridinedicarboxamide （BAP） with DNA was studied by agarose gel electrophoresis analysis. The results indicate that the BAP-Cu^2＋ complex can promote the cleavage of phosphodiester bond of supercoiled DNA at physiological condition, which is 3.2 × 10^6 times higher than DNA natural degradation. A hydrolytic cleaving mechanism through the cooperation of copper ions and functional amino groups was proposed.
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| 关 键 词： | 脱氧核糖核酸分裂 磷酸二酯酶结合物 电泳 核酸酶模式 铜 |
| 修稿时间： | 2006-12-092007-03-27 |
DNA Cleavage Promoted by Cu^2＋ Complex of N,N＇-Bis（2-aminoethyl）-2,6-pyridinedicarboxamide |
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| LI Ying, SHENG Xin, SHAO Ying, LU Guo-Yuan.DNA Cleavage Promoted by Cu^2＋ Complex of N,N＇-Bis（2-aminoethyl）-2,6-pyridinedicarboxamide[J].Chinese Journal of Chemistry,2007,25(7):937-941. |
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| Authors: | LI Ying SHENG Xin SHAO Ying LU Guo-Yuan |
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| Institution: | Department of Chemistry, State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing, Jiangsu 210093, China |
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| Abstract: | The interaction of Cu2+ complex of N,N′‐bis(2‐aminoethyl)‐2,6‐pyridinedicarboxamide (BAP) with DNA was studied by agarose gel electrophoresis analysis. The results indicate that the BAP‐Cu2+ complex can promote the cleavage of phosphodiester bond of supercoiled DNA at physiological condition, which is 3.2×106 times higher than DNA natural degradation. A hydrolytic cleaving mechanism through the cooperation of copper ions and functional amino groups was proposed. |
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| Keywords: | DNA cleavage phosphodiester bond electrophoresis nuclease model |
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