| Simultaneous Determination of Epinephrine, Noradrenaline and Dopamine in Human Serum Samples by High Performance Liquid Chromatography with Chemiluminescence Detection |
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| 作者姓名： | 陈福南 张迎雪 章竹君 |
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| 作者单位： | [1]College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China [2]Department of Chemistry, Qiongzhou University, Qiongzhou, Hainan 572200, China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China （No. 20175039）. |
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| 摘 要： | A simple, rapid and accurate high performance liquid chromatographic （HPLC） technique coupled with chemiluminescence （CL） detection was developed for the simultaneous determination of epinephrine （E）, noradrenaline （NA） and dopamine （DA）. It was based on the analyte enhancement effect on the CL reaction between luminol and potassium ferricyanide. The effects of various parameters, such as potassium ferricyanide concentration, luminol concentration, pH value and component of the mobile phase on chromatographic behaviors of the analytes （E, NA and DA） were investigated. The separation was carded out on C18 column using the mobile phase of 0.01 mol/L potassium hydrogen phthalate solution and methanol （92 ： 8, V/V）. Under the optimum condi- tions, E, NA and DA showed good linear relationships in the range of 1 × 10^-8 -5 × 10^-6, 5.0× 10^-9 -1.0× 10^-6 and 5.0×10^-9-1.0× 10^-6 g]mL respectively. The detection limits for E, NA and DA were 4.0×10^-9, 1.0× 10^-9 and 8.0 × 10^-10 g/mL. The proposed method has been applied successfully to the analysis of E, NA and DA in human serum samples.
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| 关 键 词： | 高性能液体色谱法 化学发光 去甲肾上腺素 多巴胺 血液样本 |
| 修稿时间： | 2006-09-262007-03-22 |
Simultaneous Determination of Epinephrine, Noradrenaline and Dopamine in Human Serum Samples by High Performance Liquid Chromatography with Chemiluminescence Detection |
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| CHEN Fu-Nana, ZHANG Ying-Xue, ZHANG Zhu-Jun.Simultaneous Determination of Epinephrine, Noradrenaline and Dopamine in Human Serum Samples by High Performance Liquid Chromatography with Chemiluminescence Detection[J].Chinese Journal of Chemistry,2007,25(7):942-946. |
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| Authors: | CHEN Fu-Nana ZHANG Ying-Xue ZHANG Zhu-Jun |
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| Institution: | 1. College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China ;2. Department of Chemistry, Qiongzhou University, Qiongzhou, Hainan 572200, China |
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| Abstract: | A simple, rapid and accurate high performance liquid chromatographic (HPLC) technique coupled with chemiluminescence (CL) detection was developed for the simultaneous determination of epinephrine (E), noradrenaline (NA) and dopamine (DA). It was based on the analyte enhancement effect on the CL reaction between luminol and potassium ferricyanide. The effects of various parameters, such as potassium ferricyanide concentration, luminol concentration, pH value and component of the mobile phase on chromatographic behaviors of the analytes (E, NA and DA) were investigated. The separation was carried out on C18 column using the mobile phase of 0.01 mol/L potassium hydrogen phthalate solution and methanol (92:8, V/V). Under the optimum conditions, E, NA and DA showed good linear relationships in the range of 1×10?8–5×10?6, 5.0×10?9–1.0×10?6 and 5.0×10?9–1.0×10?6 g/mL respectively. The detection limits for E, NA and DA were 4.0×10?9, 1.0×10?9 and 8.0×10?10 g/mL. The proposed method has been applied successfully to the analysis of E, NA and DA in human serum samples. |
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| Keywords: | high performance liquid chromatography chemiluminescence noradrenaline epinephrine dopamine |
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