Determination of nifedipine in dog plasma by high‐performance liquid chromatography with tandem mass spectrometric detection |
| |
Authors: | Xigui Pan Shunchang Zhou Qinqin Fu Xianming Hu Jianhong Wu |
| |
Affiliation: | 1. School of Pharmaceutical Science, Wuhan University, Wuhan, People's Republic of China;2. Department of Pharmacy, Remin Hospital of Wuhan University, Wuhan, People's Republic of China;3. Tongji Medical College of Huazhong University of Science and Technology, Wuhan, People's Republic of China;4. Wuhan Institute for Food and Drug Control, Wuhan, People's Republic of China |
| |
Abstract: | Nifedipine is a dihydropyridine calcium channel blocker used widely in the management of hypertension and other cardiovascular disorders. In this work, a simple, rapid and sensitive liquid chromatography/tandem mass spectrometry method was developed and validated to determine nifedipine in dog plasma using nimodipine as the internal standard. Chromatographic separation was carried out on a C8 column. The mobile phase consisted of a mixture of acetonitrile, water and formic acid (60:40:0.2, v/v/v) at a flow rate of 0.5 mL/min. Detection was performed on a triple quadrupole tandem mass spectrometer in selected reaction monitoring mode via an atmospheric pressure chemical ionization source. The method has a lower limit of quantification of 0.20 ng/mL with consumption of plasma as low as 0.05 mL. The linear calibration curves were obtained in the concentration range of 0.20–50.0 ng/mL (r = 0.9948). The recoveries of the liquid extraction method were 74.5–84.1%. Intra‐day and inter‐day precisions were 4.1–8.8 and 6.7–7.4%, respectively. The quantification was not interfered with by other plasma components and the method was applied to determine nifedipine in plasma after a single oral administration of two controlled‐release nifedipine tablets to beagle dogs. Copyright © 2013 John Wiley & Sons, Ltd. |
| |
Keywords: | nifedipine nimodipine LC‐MS/MS pharmacokinetics beagle dog |
|
|