Simultaneous determination of aurantio‐obtusin,chrysoobtusin, obtusin and 1‐desmethylobtusin in rat plasma by UHPLC‐MS/MS |
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Authors: | Wei‐dong Zhang Peng‐yuan Wang Ying Wang Qing Wang Yi Gu Jun Cao Shao‐qing Wang Xiao‐juan Wang |
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Institution: | 1. Department of Pharmacy, School of Stomatology, Fourth Military Medical University, Xi'an, People's Republic of China;2. College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, China;3. Department of Pathology, Qiqihaer Medical University, Qiqihaer, China |
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Abstract: | A sensitive and reliable ultra‐high‐performance liquid chromatography–electrospray ionization–tandem mass spectrometry (UHPLC‐MS/MS) method was developed and validated for the simultaneous determination of four active components of Semen Cassiae extract (aurantio‐obtusin, chrysoobtusin, obtusin and 1‐desmethylobtusin) in rat plasma after oral administration. Chromatographic separation was achieved on an Agilent Poroshell 120 C18 column with gradient elution using a mobile phase that consisted of acetonitrile‐ammonium acetate in water (30 mm ) at a flow rate of 0.4 mL/min. Detection was performed by a triple‐quadrupole tandem mass spectrometer in multiple reaction monitoring mode. The calibration curve was linear over a range of 3.24–1296 ng/mL for aurantio‐obtusin, 0.77–618 ng/mL for chrysoobtusin, 34.55–1818 ng/mL for obtusin and 1.86–1485 ng/mL for 1‐desmethylobtusin. Inter‐ and intra‐day assay variation was <15%. All analytes were shown to be stable during all sample storage and analysis procedures. Copyright © 2013 John Wiley & Sons, Ltd. |
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Keywords: | semen cassiae aurantio‐obtusin chrysoobtusin obtusin 1‐desmethylobtusin UHPLC‐MS/MS |
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