Pharmacokinetics and oral bioavailability of epimedin C after oral administration of epimedin C and Herba Epimedii extract in rats |
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Authors: | Chia‐Jung Lee Yu‐Tse Wu Thomas Y. Hsueh Lie‐Chwen Lin Tung‐Hu Tsai |
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Affiliation: | 1. Institute of Traditional Medicine, School of Medicine, National Yang‐Ming University, Taipei, Taiwan;2. Department of Education and Research, Taipei City Hospital, Taipei, Taiwan;3. School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan;4. Department of Urology, School of Medicine, National Yang‐Ming University, Taipei, Taiwan;5. National Research Institute of Chinese Medicine, Taipei, Taiwan;6. Graduate Institute of Acupuncture Science, China Medical University, Taichung, Taiwan |
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Abstract: | Epimedin C, an ingredient of Herba Epimedii, has potential for treatment of cardiovascular disease and bone loss. However, there is still no sensitive analytical method to monitor epimedin C in biological samples. The goal of this study was to develop a sensitive and reliable method based on a LC‐MS/MS for evaluating the pharmacokinetics of epimedin C after administration of Herba Epimedii in rat. Electrospray ionization in positive‐ion mode and multiple reaction monitoring were used to identify and quantitate active components. Analytes were separated by a reverse‐phase C18 column. Liquid–liquid extraction using ethyl acetate, evaporation and reconstitution was used to plasma sample preparation. Mass transition of precursor ion → product ion pairs were monitored at m/z 823.4 → 313.1 for epimedin C and m/z 237.1 → 178.9 for carbamazepine (internal standard). A calibration curve gave good linearity (r > 0.999) over the concentration range 2.5–500 ng/mL. Pharmacokinetic data demonstrated that there was rapid distribution and slow elimination after epimedin C administration (1 mg/kg, i.v.). Oral bioavailabilities of epimedin C in the pure compound and in the Herba Epimedii were around 0.58% and 0.13%, respectively. The result suggests that other herbal ingredients of Herba Epimedii may suppress the oral bioavailability of epimedin C. Copyright © 2013 John Wiley & Sons, Ltd. |
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Keywords: | bioavailability epimedin C Epimedium davidii Franch herbal medicine traditional Chinese medicine |
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