Contribution of cell culture additives to the two-dimensional protein patterns of mouse macrophages |
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Authors: | Miller Ingrid Radwan Marta Strobl Birgit Müller Mathias Gemeiner Manfred |
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Institution: | Institute of Medical Chemistry, University of Veterinary Medicine, Vienna, Austria. ingrid.miller@vu-wien.ac.at |
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Abstract: | Low levels of fetal calf serum (FCS), used as protein supplement in cell culture medium, were traced in preparations of primary murine macrophages (bone-marrow-derived macrophages (BMM) and peritoneal macrophages (PM)). Main components of this common additive were mapped in 2-DE by means of differential image gel electrophoresis and immunoblotting. Additional washing steps in cell preparation helped to decrease the levels of the four highest abundance foetal serum proteins (serum albumin (SA), alpha1-fetoprotein (AFP), alpha1-antitrypsin (alpha1AT) and transferrin (Tf)) to less than 1% of total protein. Macrophage spot pattern was recorded in parallel and showed little variation. Results presented are supposed to be of general interest for cell preparations with similar background. |
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