Analysis of steroid hormones in effluents of wastewater treatment plants by liquid chromatography-tandem mass spectrometry |
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Authors: | Ingrand Valerie Herry Gaëla Beausse Johanne de Roubin Marie-Renée |
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Affiliation: | Anjou Recherche-Vivendi Water, Emerging Parameters, 1 Place de Turenne, Immeuble Le Dufy, 94417 Saint Maurice Cedex, France. valerie.ingrand@generale-des-eaux.net |
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Abstract: | This paper presents the development of an analytical procedure for the determination of two sexual steroid hormones: 17beta-estradiol and estrone, and the synthetic contraceptive estrogen, 17alpha-ethynylestradiol in effluents of wastewater treatment plants. Samples are extracted via solid-phase extraction using C18 cartridges. Extracts in ethyl acetate are then purified with a liquid-liquid separation with aqueous sodium chloride, then with a clean-up on a Florisil cartridge. Steroids are analyzed using an LC-MS-MS ion trap system. Internal quantification with the corresponding deuterated steroids leads to limits of quantification at 5 ng/l for estrone and 10 ng/l for estradiol and ethynylestradiol. In mineral spiked water, recoveries are 91% for 17beta-estradiol, 97% for estrone and 87% for 17alpha-ethynylestradiol and RSDs are 15% for 17beta-estradiol, 11% for estrone and 23% for 17alpha-ethynylestradiol. |
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Keywords: | Water analysis Steroids |
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