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Direct electron transfer of horseradish peroxidase and its electrocatalysis based on carbon nanotube/thionine/gold composites
Affiliation:1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Graduate University of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun 130022, PR China;2. Laboratory of Analytical Chemistry, Process Chemistry Centre, Åbo Akademi University, Biskopsgatan 8, FI-20500 Åbo-Turku, Finland
Abstract:Horseradish peroxidase (HRP) was incorporated into multiwalled carbon nanotube/thionine/Au (MTAu) composite film by electrostatic interactions between positively charged HRP and negatively charged MTAu composite. The results of electrochemical impedance spectroscopy (EIS) confirmed adsorption of HRP on the surface of MTAu modified GC electrode. Moreover, the electrochemical results showed that HRP retained its bioactivity and bioelectrocatalytical activity, and also showed good direct electron transfer behavior on such a composite film.
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