Characterization of polysaccharide production of Haemophilus influenzae type b and its relationship to bacterial cell growth |
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Authors: | Mickie Takagi Joaquin Cabrera-Crespo Júlia Baruque-Ramos Teresa Cristina Zangirolami Isaias Raw Martha Massako Tanizaki |
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Institution: | 1. Laboratório de Fermenta??o Instituto Butantan, Centro de Biotecnologia, Av. Vital Brasil, 1500, CEP 05503-900, S?o Paulo, Brazil 2. Laboratório de Fermenta??o Departmento de Engenharia Química, Universidade Federal de S?o Carlos, Via Washington Luiz, 235, CEP 13565-905, S?o Carlos, SP, Brazil
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Abstract: | Haemophilus influenzae type b (Hib) causes invasive infections in infants and young children. Vaccines consisting of Hib capsular polysaccharide
(polymer of ribosylribitol phosphate PRP]) conjugated to a protein are effective in the prevention of such infections. The
production of capsular polysaccharide type b was studied in three cultivation conditions: single, glucose pulse, and repeated
batch. Specific polysaccharide production (Y
p/x
) was calculated for all experiments, showing the following values: 67 (single-batch cultivation), 71 (glucose pulse), 75
(repeated-batch cultivation, first batch), and 87 mg of PRP/g of dry cell weight (DCW) (repeated-batch cultivation, second
batch). Biomass concentration reached ∼1.8 g of DCW/L, while polysaccharide concentration was about ∼132 mg/L in the three
fermentation runs. Polysaccharide synthesis is associated with cell growth in all studied conditions as established by Kono's
analysis and Luedeking-Piret's model. |
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Keywords: | Haemophilus influenzae capsular polysaccharide polymer of ribosylribitol phosphate batch cultivation |
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