| 反相高效液相色谱法测定肉松制品中姜黄素的含量 |
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| 引用本文： | 蔡小明.反相高效液相色谱法测定肉松制品中姜黄素的含量[J].广州化学,2014(3):7-10. |
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| 作者姓名： | 蔡小明 |
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| 作者单位： | 福建省产品质量检验研究院国家加工食品质量监督检验中心,福建福州350002 |
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| 摘 要： | 建立了肉松制品中姜黄素含量的反相高效液相色谱测定方法。35℃下,以Ultimate XB-C18（4.6mm×150 mm,5μm）为分析柱,乙腈和0.1%磷酸水溶液（pH2.2）作流动相（体积比为50∶50）等度洗脱,流速1.0 mL/min,425 nm波长下检测。结果表明姜黄素在1.69~169μg/mL浓度范围内呈良好的线性关系,线性回归系数r=0.999 9,方法检出限为1.0 mg/kg,实际样品加标回收率范围为86.9%~94.5%,相对标准偏差为4.6%~7.8%（n=6）。该方法准确可靠、重复性好,可用于肉松制品中姜黄素含量测定。
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| 关 键 词： | 姜黄素 肉松 高效液相色谱 |
Determination of Curcumin in Dried Meat Floss by RP-HPLC |
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| CAI Xiao-ming.Determination of Curcumin in Dried Meat Floss by RP-HPLC[J].Guangzhou Chemistry,2014(3):7-10. |
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| Authors: | CAI Xiao-ming |
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| Institution: | CAI Xiao-ming ( 1. Fujian Inspection and Research Institute for Product Quality, China National Quality Supervision and Testing Center for Processed Food, Fuzhou 350002, China) |
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| Abstract: | A method of high performance liquid chromatography （HPLC） was developed for the separation and determination of curcumin in Dried Meat Floss. The HPLC separation was carried out on aUltimate XB-C18 column （4.6 mm× 150 mm, 5 μm）. A mixture of acetonitrile and 0.1% phosphoric acid solution （pH 2.2） （50 ： 50, V/V） was used as mobile phase at a flow rate of 1.0 mL/min. The column temperature was kept below 35℃, and the detection wavelength was set at 425 nm. The linear range was 1.69-169 μg/mL, a good correlation coefficient was obtained, r=0.999 9. Satisfactory recoveries （86.9%~94.5% for methanol） were achieved, and RSD were 4.6%~7.8% （n=6）. The method was proved to be simple and accurate, and has been applied in the determination of curcumin in dried meat floss. |
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| Keywords: | curcumin dried meat floss high performance liquid chromatography （HPLC） |
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