| 基于固定化酶的化学发光停流法测定D-氨基酸 |
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| 引用本文： | 封满良,黄玉文,宫志龙,章竹君.基于固定化酶的化学发光停流法测定D-氨基酸[J].高等学校化学学报,1996,17(12):1859. |
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| 作者姓名： | 封满良 黄玉文 宫志龙 章竹君 |
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| 作者单位： | 陕西师范大学化学系, 西安, 710062 |
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| 摘 要： | 基于固定化酶的化学发光停流法测定Ｄ－氨基酸封满良，黄玉文，宫志龙，章竹君（陕西师范大学化学系，西安，７１００６２）关键词固定化酶，化学发光，甲壳质，Ｄ－氨基酸自从发现人体有关组织及血桨中Ｄ－氨基酸的水平与某些疾病有关以来，已报道了许多测定Ｄ－氨基酸的...
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| 关 键 词： | 固定化酶 化学发光 甲壳质 D-氨基酸 |
| 收稿时间： | 1995-12-13 |
Chemiluminescence Determination of D-amino Acid Based on Immobilized Enzymes |
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| FENG Man-Liang,HUANG Yu-Wen,GONG Zhi-Long,ZHANG Zhu-Jun.Chemiluminescence Determination of D-amino Acid Based on Immobilized Enzymes[J].Chemical Research In Chinese Universities,1996,17(12):1859. |
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| Authors: | FENG Man-Liang HUANG Yu-Wen GONG Zhi-Long ZHANG Zhu-Jun |
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| Institution: | Department of Chemistry, Shaanxi Nornzal University, Xi'an, 710062 |
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| Abstract: | D-amino acid oxidase and HRPwere separately immobilized on chitosan adsorbed on the silica gels to develop a chemiluminescent method,which was combined with two oxidation reactions of D-amino acid producing H2O2 catalyzed by D-amino acid oxidase and of Luminol-H2O2 generating CLcatalyzed by HRPtogether,also was stopped──flow technique used in this system. It has a good selectivity and sensitivity. Under optimized conditions,the detection limit is 1.8 ×10-5g/mL,the liner range of D-amino acid concentration was from 8× 10-8 to 1. 4×10-6g/mL,It was practicable to determine D-amino acid in serum directly with satisfactory results. |
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| Keywords: | Immobilized enzymes CL Chitosan D-amino acid |
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