2'-deoxycytidines carrying amino and thiol functionality: synthesis and incorporation by Vent (exo-) polymerase |
| |
Authors: | Roychowdhury Abhijit Illangkoon Heshan Hendrickson Cynthia L Benner Steven A |
| |
Institution: | Department of Chemistry, University of Florida, P.O. Box 117200, Gainesville, Florida 32611, USA. |
| |
Abstract: | reaction: see text] The synthesis of 2'-deoxycytidine nucleosides bearing amino and thiol groups appended to the 5-position of the nucleobase via a butynyl linker is described. The corresponding triphosphates were then synthesized from the nucleoside and incorporated into oligonucleotides by Vent (exo(-)) DNA polymerase. The ability of Vent (exo(-)) polymerase to amplify oligonucleotides containing these functionalized cytidine derivatives in a polymerase chain reaction (PCR) was demonstrated for the amino-functionalized derivative. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|