Dopamine and iron mediated fragmentation of galactocerebroside and cardiolipin in micelles |
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Authors: | Irina Yurkova Mikhail Kisel Juergen Arnhold Oleg Shadyro |
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Institution: | 1. Research Institute for Physical Chemical Problems of the Belarusian State University, Leningradskaya str. 14, 220050, Minsk, Belarus 2. Institute of Bioorganic Chemistry, Belarus National Academy of Sciences, Kuprevicha str. 5/2, 220080, Minsk, Belarus 3. Institute of Medical Physics and Biophysics, Medical Department, University of Leipzig, H?rtelstr. 16-18, D-04107, Leipzig, Germany
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Abstract: | Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and thin-layer chromatography
(TLC) have been used to study dopamine and iron mediated free-radical transformation of lipids in their hydrophilic parts.
It has been shown that the action of the dopamine/Fe2+ system on galactocerebroside or cardiolipin, which are the components of mixed micelles, results in formation of ceramide
or phosphatidic acid and phosphatidylhydroxyacetone, respectively. These data, when combined with results obtained using the
ascorbate/Fe2+/H2O2 oxidizing system with the same substrates, demonstrate that the formation of these products proceeds via an OH-radical induced
fragmentation taking place in polar moiety of the starting lipids.
![MediaObjects/11532_2007_Article_41_Fig1_HTML.jpg](/content/7638331506443370/MediaObjects/11532_2007_Article_41_Fig1_HTML.jpg) |
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Keywords: | free-radical fragmentation lipids MALDI-TOF MS thin-layer chromatography |
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