| 荧光物质的酯类衍生物用作人血胆碱酯酶底物的研究 |
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| 引用本文： | 杨梅,张素玢,冯立娟.荧光物质的酯类衍生物用作人血胆碱酯酶底物的研究[J].辽宁师范大学学报(自然科学版),2001,24(1):51-53. |
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| 作者姓名： | 杨梅 张素玢 冯立娟 |
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| 作者单位： | 1. 辽宁师范大学 化学系,
2. 葫芦岛 市广播电视大学, |
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| 基金项目： | 辽宁省教育厅高校科研项目(9802211016) |
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| 摘 要： | 合成了几种荧光物质4－甲基伞形酮、荧光素、二溴荧光素的乙酸酯及丁酸酯，研究了它们用作人血胆碱酯酶（ChE）底物进行活性测定的各种条件，通过米氏常数（Km）和人血ChE稀释比曲线的测定系统地比较了六种物质作为底物与ChE作用的特异性和灵敏度。结果表明荧光物质量子产率越高、发射波长越长、酯基的烃基链越长的物质，用作人血ChE活性测定底物的选择性和灵敏度越好。
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| 关 键 词： | 胆碱酯酶 底物 荧光物质 酯类衍生物 活性测定 米氏常数 人血 荧光强度 |
| 文章编号： | 1000-1735（2001）01-0051-03 |
| 修稿时间： | 2000年11月10 |
The Study of the Ester
Deriviatives of Florescent Substances Used as the Substrate of Cholinesterase in Human
Blood |
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| YANG Mei,ZHANG Su-bin,FENG Li-juan.The Study of the Ester
Deriviatives of Florescent Substances Used as the Substrate of Cholinesterase in Human
Blood[J].Journal of Liaoning Normal University(Natural Science Edition),2001,24(1):51-53. |
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| Authors: | YANG Mei ZHANG Su-bin FENG Li-juan |
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| Abstract: | In this paper, we choose several qualified fluorescent subtances: 4 - methylumbelliferyl, fluorescein, 3, 7dibromo-fluorescein, synthetised their acetate and butyrate, studied the feasibility of them used as substrates for the determination of cholinesterase(ChE). Meanwhile, by Km and ChE's diluted ratio curve, the sensitivity and selectivity of these substrates are evalutated and compared. The results show that the esters of those fluorescent substance possess higher quantium yield and longer emission wavelength with better substrate of Che. |
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| Keywords: | fluorescence substrate cholinesterase |
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