Determination of 8-hydroxyquinoline sulfate in tuberculin solutions by planar and high performance liquid chromatography |
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| Authors: | D Agbaba A Mihajlovic P Beljanski D Zivanov-Stakic S Vladimirov |
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| Institution: | (1) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Vojvode Stepe 450, P.O. Box 146, 11000 Belgrade, Serbia (Yugoslavia);(2) Torlak-Institute of Immunology and Virology, Vojvode Stepe 458, 11221 Belgrade, Serbia (Yugoslavia) |
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| Abstract: | Summary TLC and HPLC methods for the determination of the preservative, 8-hydroxyquinoline sulfate in PPD-T tuberculin solution were
developed. The planar chromatography method involved separation of 8-hydroxyquinoline sulfate on a TLC plate using a butyl-acetate:
formic acid: 2-propanol mobile phase, detection and quantitation by densitometric scanning. The HPLC method was on a LiChrosorb
RP-18 column with acetonitrile-water (65:35 v/v) mobile phase, adjusted to pH 3.05 by phosphoric acid. Linearity, reproducibility
and accuracy were found to be satisfactory. Under selected conditions, the limit of detection (LOD) of both methods was similar-about
25 ng.
Presented at the 21st ISC held in Stuttgart, Germany, 15th–20th September, 1996 |
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| Keywords: | Thin-layer chromatography Column liquid chromatography PPD-tuberculin solution 8-Hydroxyquinoline sulfate-preservative |
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