人参与附子、黄连配伍的HPLC-ESI-MS研究及抗氧化活性测定

采用高效液相色谱与电喷雾质谱联用技术(HPLC-ESI-MS)对不同比例的人参与附子或黄连的水煎液、药渣和沉淀进行研究, 共鉴定了8种人参皂苷, 发现加入附子或黄连后, 溶液中人参皂苷含量明显减少, 进一步研究证明附子或黄连中的某些成分阻止了皂苷的溶出; 同时以抗坏血酸作对照, 应用铁离子还原/抗氧化能力测定法(FRAP)测定了人参单煎液及人参与附子、黄连共煎液中正丁醇提取物和水提取物的抗氧化活性. 结果证明, 共煎液中正丁醇提取物的抗氧化活性高于人参单煎液, 同时也高于人参与附子或黄连单煎混合液的抗氧化活性, 但水提取物抗氧化活性的变化规律有所不同, 这可能与煎煮过程中物质之间的相互作用有关.

HPLC-ESI-MS Analysis of Ginsenosides and Antioxidant Activity During Combination of Ginseng with Aconitum Carmichaeli and Coptis Chinensis

Eight ginsenosides in ginseng, uncombined and combined with Aconitum carmichaeli or Coptis chinensis, have been investigated by high performance liquid chromatography(HPLC) and electrospray ionization-mass spectrometry(ESI-MS). The experimental results demonstrated that the contents of ginsenosides decreased in the decoction of ginseng with Aconitum carmichaeli or Coptis chinensis, and the stripping of ginsenosides was stained during the combination. Furthermore, the antioxidant activity of n-butanol extract in compatible decoction was not only better than the single decoction of ginseng, but also higher than the mixture of the single decoction of ginseng, Aconitum carmichaeli and Coptis chinensis, while the variation of water extract was different, which was determined by ferric reducing antioxidant power(FRAP) method. That might be related to the interaction during the decoction process.