| 三维同步偏振荧光光谱研究蛋白质溶液变性时氨基酸残基的发光行为 |
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| 引用本文： | 唐波,杜鸣,陈蓁蓁,张慧,沈含熙.三维同步偏振荧光光谱研究蛋白质溶液变性时氨基酸残基的发光行为[J].化学学报,2004,62(12):1153-1157,MJ04. |
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| 作者姓名： | 唐波 杜鸣 陈蓁蓁 张慧 沈含熙 |
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| 作者单位： | 山东师范大学化学化工与材料科学学院,济南,250014;南开大学化学系,天津,300071 |
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| 基金项目： | 国家自然科学基金 (No.2 0 3350 30 )，山东省自然科学基金 (No.Z2 0 0 3B0 1 )资助项目 |
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| 摘 要： | 将偏振技术、同步技术与三维技术结合起来的三维同步偏振荧光光谱(TDSPS)能分辨蛋白质溶液中的色氨酸(Trp)和酪氨酸(Tyr)残基，具有同步光谱分辨率高、三维技术信息丰富的优点．本文用TDSPS表征牛血清白蛋白(BSA)、人血清白蛋白(HSA)受各种因素影响(酸效应、碱效应、盐效应、猝灭剂等)时Trp，Tyr残基荧光光谱的变化，用于区分HSA和BSA．
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| 关 键 词： | 三维同步偏振荧光光谱 色氨酸 酪氨酸 牛血清白蛋白 人血清白蛋白 |
Studies on Luminescence of Trp and Tyr Residues in Protein Denaturation by Three-dimensional-synchronous-polarized Spectrofluorimetry |
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| TANG,Bo,a DU,Ming a CHEN,Zhen-Zhen a ZHANG,Hui a SHEN,Han-Xi b.Studies on Luminescence of Trp and Tyr Residues in Protein Denaturation by Three-dimensional-synchronous-polarized Spectrofluorimetry[J].Acta Chimica Sinica,2004,62(12):1153-1157,MJ04. |
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| Authors: | TANG Bo a DU Ming a CHEN Zhen-Zhen a ZHANG Hui a SHEN Han-Xi b |
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| Abstract: | Based on polarizing, synchronous and three-dimensional technologies, a new three-dimensional- synchronous-polarized spectrofluorimetry (TDSPS) was used to distinguish tryptophan (Trp) and tyrosine (Tyr) residues in albumin solution with the advantages of abundant information of three-dimensional technology and high resolution of synchronous spectrofluorimetry. In this paper, the fluorescent spectral changes of Tyr and Trp residues in bovine serum albumin (BSA) and human serum albumin (HSA) were studied by obtaining the characteristic information that had been influenced by various factors (acidic, basic, salt and quenching reagents effects, etc.) with TDSPS. As a result, HSA and BSA could be distinguished. |
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| Keywords: | three-dimensional-synchronous-polarized spectrofluorimetry (TDSPS) tryptophan (Trp) tyrosine (Tyr) bovine serum albumin (BSA) human serum albumin (HSA) |
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