Presence of a dipeptidyl aminopeptidase III in Saccharomyces cerevisiae |
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| Authors: | Y Watanabe Y Kumagai Y Fujimoto |
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| Affiliation: | Department of Clinical Biochemistry, Hokkaido Institute of Pharmaceutical Sciences, Japan. |
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| Abstract: | The soluble fraction of a cell extract of Saccharomyces cerevisiae was found to contain hydrolytic activity toward arginyl-arginyl-beta-naphthylamide (Arg-Arg-beta NA). Most of the contaminating Arg-beta NA hydrolysis activity was removed by diethylaminoethyl (DEAE)-cellulose column chromatography and the enzyme was partially purified. It is very similar in various properties to mammalian dipeptidyl aminopeptidase III (DAP III), although it differs slightly in some respects. |
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