GC-MS-SIM quantitation of amantadine in biological samples

Summary A simple, selective and sensitive method for the quantitation of amantadine in biological samples is described. After extraction from biological samples using a two-step procedure, amantadine is quantitatively converted into its isothiocyanate (NCS) derivative by treatment with carbon disulfide and then determined quantitatively using isobaric compounds, p-methoxyphenyl-ethylamine-NCS or N-acetylamantadine as external standards. The applicability of this method is illustrated by determining plasma levels of amantadine for pharmacokinetic studies and levels in dialysate samples of amantadine treated patients who were on dialysis machines.