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On-column sample enrichment for the high-sensitivity sheath-flow CE-MS analysis of peptides |
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| Authors: | K. Sandra F. Lynen B. Devreese J. Van Beeumen P. Sandra |
| Affiliation: | (1) Laboratory of Protein Biochemistry and Protein Engineering, Ghent University, K.L. Ledeganckstraat 35, 9000 Gent, Belgium;(2) Pfizer Analytical Research Centre (PARC), Ghent University, 9000 Ghent, Belgium;(3) Present address: Peakadilly nv., Technologiepark 4, VIB Bio-incubator, 9052 Zwijnaarde/Ghent, Belgium |
| Abstract: | A sample enrichment technique to increase sensitivity in capillary electrophoresis–mass spectrometry (CE-MS) is described. Peptides or glycopeptides are retained and concentrated on a short (3–5-mm) reversed-phase (C18) packed-bed situated in the fused-silica separation capillary and are subsequently released for electrophoretic separation by injection of an organic elutant. The concentration limits of detection are in the high picomolar range with a sheath-flow CE-MS interface. |
| Keywords: | Capillary electrophoresis Mass spectrometry Peptides In-line preconcentration |
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