Investigation of novel mediators for a glucose biosensor based on metal picolinate complexes |
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Authors: | Warren Susan McCormac Timothy Dempsey Eithne |
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Institution: | Electrochemical Technology Research Centre, Dept. Applied Science, Institute of Technology Tallaght, Tallaght, Dublin 24, Ireland. |
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Abstract: | The metal complexes Os(byp)(2)(pic)](+) and Ru(byp)(2)(pic)](+) where byp is 2,2'-bipyridine and HPic is o-picolinic acid were synthesised and characterised using spectroscopic and electrochemical techniques. These complexes were then evaluated as mediators for a glucose oxidase (GOx)-based biosensor. Results demonstrate the electrocatalytic behaviour of both metal couples towards regeneration of the flavoprotein GOx (FADH(2)) group, when co-immobilised with glucose oxidase. Surface immobilisation was achieved by potential cycling in aqueous solutions of the metal complexes at a glucose oxidase (GOx)/Nafion modified electrode. This proved successful in terms of catalytic efficiency and stability of redox sites. Kinetic parameters associated with both enzymatic and mediator reactions were estimated and the stability/performance properties of the sensor were tested. |
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