Optimization of Oligosaccharide Synthesis from Cellobiose by Dextransucrase |
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Authors: | Misook Kim Donal F Day |
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Institution: | (1) Department of Food Science, Louisiana State University, Baton Rouge, LA 70803, USA;(2) Audubon Sugar Institute, St. Gabriel, LA 70776, USA |
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Abstract: | There is a growing market for oligosaccharides as sweeteners, prebiotics, anticariogenic compounds, and immunostimulating
agents in both food and pharmaceutical industries. Interest in novel carbohydrate-based products has grown because of their
reduced toxicity and low immune response. Cellobiose is potentially valuable as a nondigestible sugar. The reaction of cellobiose,
as an acceptor with a sucrose as a donor, catalyzed by a dextransucrase from Leuconostoc mesenteroides B-512FMCM, produced a series of cellobio-oligosaccharides. This production system was optimized using a Box–Behnken experimental
design for 289 mM of sucrose and 250 mM of cellobiose and 54 U of the enzyme at pH 5.2 and 30 °C, to produce maximum yields
of oligosaccharide. |
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Keywords: | Oligosaccharide Cellobiose Dextransucrase Leuconostoc mesenteroides B-512FMCM Box-Behnken experimental design |
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