Performance Validation for Microplate Fluorimeters |
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Authors: | Email author" target="_blank">Robert?GiebelerEmail author Evelyn?McGown Todd?French John?C?Owicki |
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Institution: | (1) Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, California, 94089;(2) Owicki Consulting, 956 N. California Avenue, Palo Alto, California, 94303 |
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Abstract: | As the sophistication of instruments that make fluorimetric measurements on samples in microplates has increased, so has the need for methods to validate instrumental performance. This paper describes a solid-state validation microplate that tests multiple aspects of fluorescence performance, including signal linearity, gain, noise, sensitivity, wavelength accuracy, and polarization stability. Both the operating principles and the validation of the validation microplate are discussed. |
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Keywords: | Validation microplate fluorescence intensity wavelength |
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