| 鹌鹑和黑斑蛙核糖体蛋白L15 cDNA片段的克隆和序列分析 |
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| 引用本文： | 张竞男,宋平,彭茂宇,吕道远,陈云贵,周伟,陈春红. 鹌鹑和黑斑蛙核糖体蛋白L15 cDNA片段的克隆和序列分析[J]. 武汉大学学报(理学版), 2003, 49(6): 751-755 |
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| 作者姓名： | 张竞男 宋平 彭茂宇 吕道远 陈云贵 周伟 陈春红 |
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| 作者单位： | 武汉大学,生命科学学院,湖北,武汉,430072 |
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| 基金项目： | 国家自然科学基金资助项目(30150005) |
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| 摘 要： | 通过R-PCR方法、分别从两栖类的黑斑蛙(Rana nigromaculata)和鸟类的鹌鹑(Coturnix coturnix japonica)中首次克隆了核糖体大亚基蛋白Ll5(RPL15ribosomal protein L15)的cDNA片段,并分析了其序列特征,黑斑蛙和鹌鹑的rpll5cDNA片段分别为467个核苷酸和408个核苷酸，推测分别编码155个氨基酸和136个氨基酸的蛋白片段，与其它已知的脊椎动物，rpl15序列有高度的同源性．应用这两条cDNA序列和其它已知的脊椎动物rpll5序列构建的系统发育树显示聚类结果与传统分类一致。
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| 关 键 词： | 黑斑蛙 鹌鹑 核糖体蛋白L15 序列分析 系统发育树 基因克隆 |
| 文章编号： | 1671-8836(2003)06-0751-05 |
| 修稿时间： | 2003-06-25 |
Molecular Cloning and Sequence Analysis of Ribosomal Protein L15 (RPL15) cDNA Fragments Respectively from Coturnix coturnix japonica and Rana nigromaculata |
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| ZHANG Jing-nan,SONG Ping,PENG Mao-yu,LU Dao-yuan,CHEN Yun-gui,ZHOU Wei,CHEN Chun-hong. Molecular Cloning and Sequence Analysis of Ribosomal Protein L15 (RPL15) cDNA Fragments Respectively from Coturnix coturnix japonica and Rana nigromaculata[J]. JOurnal of Wuhan University:Natural Science Edition, 2003, 49(6): 751-755 |
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| Authors: | ZHANG Jing-nan SONG Ping PENG Mao-yu LU Dao-yuan CHEN Yun-gui ZHOU Wei CHEN Chun-hong |
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| Abstract: | The cDNA fragments of ribosomal protein L15 (RPL15) were cloned respectively from Rana nigromaculata and Coturnix coturnix japonica by RT-PCR. The two cDNA fragments are individually composed of 467 bp and 408 bp, encoding a protein fragment of 155 aa and another one of 136 aa. Alignment analysis indicated that both the two nucleotide sequences and the deduced amino acid sequences show high identity to other known rpl15 sequences from vertebrates. The two cDNA sequences were used to construct phylogenetic trees with other known vertebrate rpl15 sequences, and the obtained trees demonstrated similar topology with the classical systematics. |
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| Keywords: | Rana nigromaculata Coturnix coturnix japonica ribosomal protein L15(RPL15) sequence analysis phylogeny |
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