Measurement of long-range forces on a single yeast cell using a gradient optical trap and evanescent wave light scattering |
| |
Authors: | Jeffrey M Sharp Aaron R Clapp Richard B Dickinson |
| |
Institution: | Department of Chemical Engineering, University of Florida, PO Box 116005, Gainesville, FL 32611, USA |
| |
Abstract: | The long-range equilibrium and viscous interaction forces between a single Candida albicans cell and a flat surface have been measured using a gradient optical trap as a force transducer and evanescent wave light scattering (EWLS) to determine the separation distance. In this technique the trapped cell is probed against the surface by moving the focal point of the optical trap, the equilibrium force is determined by the deflection of the most probable cell position from the trap center, and the viscous forces are determined from the relaxation time of the Brownian fluctuations of the cell in the trap. At low electrolyte concentrations (0.5 mM NaCl) where double layer repulsion was anticipated to be the dominant interaction, equilibrium force–distance profiles for yeast cells and similarly sized polystyrene microspheres on glass surfaces both showed good agreement with predictions of DLVO theory. Also, viscous drag profiles at larger separation distances where interaction forces were small agreed well with Stokes flow predictions. These results appear to validate the technique for use with spherical yeast cells and other bioparticles of similar size. This force measurement methodology therefore provides a complementary alternative to atomic force microscopy for direct force measurement with much greater sensitivity for studying interaction between yeast and surfaces. |
| |
Keywords: | Optical trap Force measurement Candida albicans Yeast adhesion |
本文献已被 ScienceDirect 等数据库收录! |
|