Aptamer-based electrochemical approach to the detection of thrombin by modification of gold nanoparticles |
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Authors: | Lidong Li Hongtao Zhao Zhengbo Chen Xiaojiao Mu Lin Guo |
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Institution: | (1) School of Chemistry and Environment, Beijing University of Aeronautics and Astronautics, Beijing, 100191, China |
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Abstract: | This paper presents a simple electrochemical approach for the detection of thrombin, using aptamer-modified electrodes. The
use of gold nanoparticles results in significant signal enhancement for subsequent detection. 1,6-Hexanedithiol was used as
the medium to link Au nanoparticles to a bare gold electrode. Anti-thrombin aptamers were immobilized on the gold nanoparticles’
surfaces by self-assembly. The packing density of aptamers was determined by cyclic voltammetric (CV) studies of redox cations
(e.g., Ru(NH3)6]3+) which were electrostatically bound to the DNA phosphate backbones. The results indicate that the total amount of aptamer
probes immobilized on the gold nanoparticle surface is sixfold higher than that on the bare electrode, leading to increased
sensitivity of the aptasensor and a detection limit of 1 pmol L−1. Based on the Langmuir model, the sensor signal displayed an almost perfect linear relationship over the range of 1 pmol
L−1 to 30 nmol L−1. Moreover, the proposed aptasensor is highly selective and stable. In summary, this biosensor is simple, highly sensitive,
and selective, which is beneficial to the ever-growing interest in fabricating portable bio-analytical devices with simple
electrical readout procedures. |
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