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Analysis of non-anthocyanin phenolic compounds in wine samples using high performance liquid chromatography with ultraviolet and fluorescence detection
Authors:Rodríguez-Bernaldo de Quirós Ana  López-Hernández Julia  Ferraces-Casais Patricia  Lage-Yusty M Asunción
Affiliation:Analytical Chemistry, Nutrition and Food Science Department, Pharmacy Faculty, University of Santiago de Compostela, Santiago de Compostela (La Coru?a), Spain. Fax: +34‐981‐594912
Abstract:A simple and rapid HPLC method with UV and fluorescence detection (FLD) for the separation of ten phenolic compounds including gallic acid, catechin, epicatechin, caffeic acid, coumaric, trans-piceid, cis-piceid, trans-resveratrol, cis-resveratrol and quercetin is reported. The UV and fluorescence detector in series provided a high selectivity for the determination of these compounds. Precisions, recoveries and LODs achieved for all the analytes were satisfactory. The proposed method was applied to the determination of these compounds in commercially available red wines.
Keywords:Chromatographic analysis  Fluorescence and UV detection  Phenolic compounds  Wine
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