Optimization of acid hydrolysis of sugarcane bagasse and investigations on its fermentability for the production of xylitol by Candida guilliermondii |
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Authors: | Rafael Fogel Rafaela Rodrigues Garcia Rebeca da Silva Oliveira Denise Neves Menchero Palacio Luciana da Silva Madeira Nei Pereira Jr. |
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Affiliation: | (1) Departmento de Engenharia Bioquímica, Escola de Química/CT, Universidade Federal do Rio de Janeiro, CEP: 21949-900 RJ, Brazil |
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Abstract: | The dilute-acid hydrolysis of sugarcane bagasse was optimized using a statistical experimental design resulting in hydrolysates containing 57.25 g/L of xylose, which were fermented with a high inoculum concentration (10 g/L of the yeast Candida guilliermondii IM/UFRJ 50088). The addition of urea reduced the time of conversion (t C) to 75 h (without nitrogen source addition t C>127 h), and, consequently, improving the rates of xylitol bioproduction. Fermentator experiments, using the optimized conditions, resulted in enhanced conversion rates, reducing t C to 30 h. The stability of the yeast in the hydrolysate was also verified in a 480-h cultivation. |
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Keywords: | Sugarcane bagasse dilute-acid hydrolysis optimization sugarcane bagasse hydrolysate xylitol bioproduction |
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