Simultaneous analysis of hydrochlorothiazide, triamterene and reserpine in rat plasma by high performance liquid chromatography and tandem solid-phase extraction |
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Authors: | Li Hang He Junting Liu Qin Huo Zhaohui Liang Si Liang Yong |
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Institution: | Institute of Analytical Chemistry, School of Chemistry and Environment, South China Normal University, Guangzhou, PR China. |
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Abstract: | A tandem solid-phase extraction method (SPE) of connecting two different cartridges (C(18) and MCX) in series was developed as the extraction procedure in this article, which provided better extraction yields (>86%) for all analytes and more appropriate sample purification from endogenous interference materials compared with a single cartridge. Analyte separation was achieved on a C(18) reversed-phase column at the wavelength of 265 nm by high-performance liquid chromatography (HPLC). The method was validated in terms of extraction yield, precision and accuracy. These assays gave mean accuracy values higher than 89% with RSD values that were always less than 3.8%. The method has been successfully applied to plasma samples from rats after oral administration of target compounds. |
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Keywords: | C18 HPLC MCX Rat plasma Tandem solid‐phase extraction |
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