Effects of Temperature and Mobile Phase Condition on Chiral Recognition of Poly(l-phenylalanine) Chiral Stationary Phase |
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Authors: | Kaname Ohyama Kana Oyamada Naoya Kishikawa Mitsuhiro Wada Yoshihito Ohba Kenichiro Nakashima Naotaka Kuroda |
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Affiliation: | 1. Department of Environmental and Pharmaceutical Sciences, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan 2. Department of Clinical Pharmacy, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan 3. Faculty of Pharmaceutical Sciences, Nagasaki International University, Nagaski, Japan
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Abstract: | Characteristics of the chiral stationary phase with poly(l-phenylalanine) peptide selector, which was in ??-helical state, was reported. Since environmental factors affect peptide conformation, the changes in enantioselectivity were examined depending on column temperature and mobile phase conditions (ionic strength, pH, mobile phase composition). Column temperature and pH drastically affected the enantioselectivity. Based on these changes, the relation between chiral recognition and secondary structure of the peptide selector was discussed. The column stability during sequential analysis under different separation conditions was also evaluated. |
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