| A New Amperometric Biosensor Based on HRP/Nano-Au/L-Cysteine/Poly（o-Aminobenzoic acid）-Membrane-Modified Platinum Electrode for the Determination of Hydrogen Peroxide |
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| 引用本文： | 唐明宇 袁若 柴雅琴. A New Amperometric Biosensor Based on HRP/Nano-Au/L-Cysteine/Poly（o-Aminobenzoic acid）-Membrane-Modified Platinum Electrode for the Determination of Hydrogen Peroxide[J]. 中国化学, 2006, 24(11): 1575-1580. DOI: 10.1002/cjoc.200690295 |
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| 作者姓名： | 唐明宇 袁若 柴雅琴 |
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| 作者单位： | Chongqing Key Laboratory of Analytical Chemistry, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China （No. 29705001）, the Chinese Education Ministry Foundation for Excellent Young Teachers （No. 2002-40）, the Natural Science Foundation of Chongqing City, China （Nos. CSTC-2004BB4149 and 2005BB4100） and High Technology Project of Southwest University （No. XSGX02）. |
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| 摘 要： | The third generation amperometric biosensor for the determination of hydrogen peroxide （H2O2） has been described. For the fabrication of biosensor, o-aminobenzoic acid （oABA） was first electropolymerized on the surface of platinum （Pt） electrode as an electrostatic repulsion layer to reject interferences. Horseradish peroxidase （HRP） absorbed by nano-scaled particulate gold （nano-Au） was immobilized on the electrode modified with polymerized o-aminobenzoic acid （poABA） with L-cysteine as a linker to prepare a biosensor for the detection of H2O2. Amperometric detection of H2O2 was realized at a potential of ＋20 mV versus SCE. The resulting biosensor exhibited fast response, excellent reproducibility and sensibility, expanded linear range and low interferences. Temperature and pH dependence and stability of the sensor were investigated. The optimal sensor gave a linear response in the range of 2.99×10^-6 to 3.55×10^-3 mol·L^-1 to H2O2 with a sensibility of 0.0177 A·L^-1·mol^-1 and a detection limit （S/N = 3） of 4.3×10^-7 mol·L^-1. The biosensor demonstrated a 95% response within less than 10 s.
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| 关 键 词： | 电流生物传感器 过氧化氢 聚（o-氨基苯酸） 纳米金 山葵过氧化物酶 铂电极 |
| 收稿时间： | 2005-12-15 |
| 修稿时间： | 2005-12-152006-03-24 |
A New Amperometric Biosensor Based on HRP/Nano‐Au/L‐Cysteine/Poly(o‐Aminobenzoic acid)‐Membrane‐Modified Platinum Electrode for the Determination of Hydrogen Peroxide |
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| TANG, Ming-Yu, YUAN, Ruo, CHAI, Ya-Qin. A New Amperometric Biosensor Based on HRP/Nano‐Au/L‐Cysteine/Poly(o‐Aminobenzoic acid)‐Membrane‐Modified Platinum Electrode for the Determination of Hydrogen Peroxide[J]. Chinese Journal of Chemistry, 2006, 24(11): 1575-1580. DOI: 10.1002/cjoc.200690295 |
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| Authors: | TANG Ming-Yu YUAN Ruo CHAI Ya-Qin |
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| Abstract: | The third generation amperometric biosensor for the determination of hydrogen peroxide (H2O2) has been described. For the fabrication of biosensor, o‐aminobenzoic acid (oABA) was first electropolymerized on the surface of platinum (Pt) electrode as an electrostatic repulsion layer to reject interferences. Horseradish peroxidase (HRP) absorbed by nano‐scaled particulate gold (nano‐Au) was immobilized on the electrode modified with polymerized o‐aminobenzoic acid (poABA) with L‐cysteine as a linker to prepare a biosensor for the detection of H2O2. Amperometric detection of H2O2 was realized at a potential of +20 mV versus SCE. The resulting biosensor exhibited fast response, excellent reproducibility and sensibility, expanded linear range and low interferences. Temperature and pH dependence and stability of the sensor were investigated. The optimal sensor gave a linear response in the range of 2.99×10?6 to 3.55×10?3 mol·L?1 to H2O2 with a sensibility of 0.0177 A·L?1·mol?1 and a detection limit (S/N=3) of 4.3×10?7 mol·L?1. The biosensor demonstrated a 95% response within less than 10 s. |
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| Keywords: | amperometric biosensor hydrogen peroxide poly(o‐aminobenzoic acid) nano‐Au horseradish peroxidase |
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