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Enzyme sensor for L-lactate using differential pulse amperometric detection |
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| Authors: | Satoshi Hikima Teiji Kakizaki and Kiyoshi Hasebel |
| Institution: | (1) Department of Chemistry, Faculty of Science, Hokkaido University, 060 Sapporo, Japan;(2) Chemical Laboratory, Iwamizawa College, Hokkaido University of Education, 068 Iwamizawa, Japan;(3) Present address: Graduate School of Environmental Earth Science, Hokkaido University, 060 Sapporo, Japan |
| Abstract: | An enzyme sensor using differential pulse (DP) amperometric detection has been developed based on the measurement of the reduction current of the oxidized form of  -nicotinamide adenine dinucleotide (NAD+) consumed by an enzyme reaction. This biosensor has the definite advantage to prevent interference caused by electrooxidative species such as ascorbate and uric acid and exhibits higher sensitivity and selectivity in comparison to the classical DC amperometric detector. The linear detection range of this biosensor was 5.0×10–5 — 5.0×10–4 mol/l and the relative standard deviation (R.S.D.) at 2.5×10–4 mol/l was 5.0%. |
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