LASER FLASH PHOTOLYSIS OF RHODOPSIN AT ROOM TEMPERATURE |
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| Authors: | R. Bensasson,E. J. Land,&dagger T. G. Truscott,&Dagger |
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| Affiliation: | *E.R. 98, Laboratoire de Chimie Physique, Universitéde Paris VI, 91405-Orsay, France.;†Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester, M20 9BX, England;‡Department of Chemistry, Paisley College, Paisley, PA1 2BE, Scotland. |
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| Abstract: | Abstract. Nanosecond flash photolysis of rhodopsin with 530 or 353 nm light produces an initial transient absorption spectrum with peaks at ˜57O and ˜420nm, and a subsequent transient species with a maximum absorption at 480 nm. These results are interpreted as the initial formation of prelumi-rhodopsin (570 nm) followed by its conversion to lumirhodopsin (470 nm). The peak at 420 nm in the first transient may be due to either hypsorhodopsin or isorhodopsin. |
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