Visualizing Cholesterol Uptake by Self‐Assembling Rhodamine B‐Labeled Polymer Inside Living Cells via FLIM‐FRET Microscopy |
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Authors: | Franziska Doll,Philipp Keckeis,Patricia Scheel,Helmut C lfen |
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Affiliation: | Franziska Doll,Philipp Keckeis,Patricia Scheel,Helmut Cölfen |
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Abstract: | Atherosclerosis is a widespread and hazardous disease characterized by the formation of arterial plaques mostly composed of fat, cholesterol, and calcium ions. The direct solubilization of cholesterol represents a promising, atheroprotective strategy to subside lipid blood levels and reverse atherosclerosis. This study deals with the in‐depth analysis of polymer‐mediated cholesterol dissolution inside living human cells. To this end, a recently described multifunctional block‐polymer is labeled with Rhodamine B (RhoB) to investigate its interaction with cells via fluorescence microscopy. This gives insight into the cellular internalization process of the polymer, which appears to be clathrin‐ and caveolae/raft‐dependent endocytosis. In cell single particle tracking reveals an active transport of RhoB polymer including structures. Förster resonance energy transfer (FRET) measurements of cells treated with a fluorophore‐tagged cholesterol derivative and the RhoB polymer indicates the uptake of cholesterol by the polymeric particles. Hence, these results present a first step toward possible applications of cholesterol‐absorbing polymers for treating atherosclerosis. |
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Keywords: | biological application of polymers fluoropolymers imaging nanoparticles self‐assembly |
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