High performance microcolumn exclusion chromatography of proteins and peptides |
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| Authors: | E S Gankina J J Kever I O Kostiuk A E Saminsky B G Belenkii |
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| Abstract: | A new method for the determination of the molecular weight of proteins and peptides has been developed. It is based on microcolumn exclusion chromatography in trifluoroacetic acid on silica gel sorbents of different porosities with a linear molecular-weight calibration dependence in the range of 5 × 102 - 7 × 104 Da. It was shown that in this eluent proteins and peptides adopt the random-coil conformation and do not undergo hydrolysis for 2–3 days at room temperature. |
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| Keywords: | Liquid chromatography Microcolumn exclusion chromatography Proteins Peptides |
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