Enzyme sensor for L-lactate with a chitosan-mercury film electrode |
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Authors: | Satoshi Hikima Teiji Kakizaki Mitsuhiko Taga and Kiyoshi Hasebe |
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Institution: | (1) Department of Chemistry, Faculty of Science, Hokkaido University, 060 Sapporo, Japan |
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Abstract: | Summary An amperometric enzyme sensor composed of a mercury film electrode and an enzyme-immobilized chitosan membrane is developed. This biosensor is based on both a mercury film electrode detecting the consumption of dissolved dioxygen following enzymatic reaction, and a chitosan membrane. The latter provides an excellent permselectivity and excludes electroactive interferents. The detection range of this biosensor was 1.0×10–5–3.0×10–4 mol/l and the relative standard deviation, R.S.D. at 5.0×10–5 mol/l was 1.4% (n=3). This biosensor was applied to the direct determination of L-lactate in human serum without pretreatment. |
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