| 2-氯化-n-氨基葡萄糖乙酰基三苯基季膦盐与DNA作用的荧光光谱研究及其应用 |
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| 引用本文： | 刘立明,蔡朝霞,宋功武,娄兆文,何瑜.2-氯化-n-氨基葡萄糖乙酰基三苯基季膦盐与DNA作用的荧光光谱研究及其应用[J].分析科学学报,2005,21(4):367-370. |
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| 作者姓名： | 刘立明 蔡朝霞 宋功武 娄兆文 何瑜 |
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| 作者单位： | 湖北大学化学与材料科学学院,武汉,430062;湖北大学化学与材料科学学院,武汉,430062;湖北大学化学与材料科学学院,武汉,430062;湖北大学化学与材料科学学院,武汉,430062;湖北大学化学与材料科学学院,武汉,430062 |
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| 摘 要： | 本文合成了2-氯化-n-氨基葡萄糖乙酰基三苯基季膦盐(2-GATPC)。利用荧光光谱法研究了2-GATPC与DNA的作用。实验结果表明,在pH3左右,DNA以静态猝灭的方式使2-GATPC的荧光强度显著降低。DNA的浓度在1.6×10-6~4.8×10-5mol.L-1的范围内,对2-GATPC的荧光有良好的线性猝灭关系,据此可以测定DNA,检出限可达8.4×10-7mol.L-1。方法简便快速,背景干扰小,对合成样品中DNA的测定,结果令人满意。
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| 关 键 词： | 2-氯化-n-氨基葡萄糖乙酰基三苯基季膦盐 荧光光谱 静态猝灭 DNA |
| 文章编号： | 1006-6144(2005)04-0367-04 |
| 收稿时间： | 2004-04-20 |
| 修稿时间： | 2004年4月20日 |
The Fluorescence Studies of Interaction of 2-(n-2'-Glucosyl) Actylramidyl Triphenyl Phosphonium Chloride with DNA and Its Application |
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| LIU Li-ming,CAI Zhao-xia,SONG Gong-wu,LOU Zhao-wen,HE Yu.The Fluorescence Studies of Interaction of 2-(n-2'-Glucosyl) Actylramidyl Triphenyl Phosphonium Chloride with DNA and Its Application[J].Journal of Analytical Science,2005,21(4):367-370. |
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| Authors: | LIU Li-ming CAI Zhao-xia SONG Gong-wu LOU Zhao-wen HE Yu |
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| Abstract: | A new compound 2-(n-2-glucosyl) actylramidyl triphenyl phosphonium chloride (2-GATPC) was synthesized,and its interaction with nucleic acid DNA was studied by fluorescence method.The results of the experiment indicated that DNA obviously decreased the fluorescence intensity of 2-GATPC by the static quenching style around pH 3.DNA could be determined by using the fluorescence quenching action of DNA on 2-GATPC.The linear range was 1.610~(-6)4.810~(-5) molL~(-1) with the detection limit of 8.410~(-7) molL~(-1) .The effects of interference substances and optimization of procedure were investigated.Three synthetic samples were determined satisfactorily. |
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| Keywords: | DNA |
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