Dynamically modified silica — the use of bare silica in reversed-phase high-performance liquid chromatography |
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| Authors: | Steen Honoré Hansen Per Helboe Mogens Thomsen |
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| Institution: | Department of Organic Chemistry, The Royal Danish School of Pharmacy, 2 Universitetsparken, DK-2100 Copenhagen, Denmark;The Drug Standardization Laboratory, National Board of Health, 378 Frederikssundsvej, DK-2700 Brønshøj, Denmark |
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| Abstract: | A new approach to reversed-phase high-performance liquid chromatography has been developed and investigated in some detail. Bare silica is used as the column packing material with an aqueous eluent containing a buffer, an organic solvent as the modifier and a long-chain quaternary ammonium ion. The eluent exhibits a high affinity to the silica surface at pH values above 5, thus, dynamically, forming a nonpolar stationary phase. |
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