Fluorescent high-performance liquid chromatography of folic acid and its derivatives using permanganate as a fluorogenic reagent |
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| Authors: | Norio Ichinose Toshihiro Tsuneyoshi Mikako Kato Tokinori Suzuki and Shigero Ikeda |
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| Institution: | (1) Department of Chemistry, Hamamatsu University School of Medicine, Handa-cho 3600, Hamamatsu-shi, 431-31 Shizuoka, Japan;(2) Shizuoka Institute of Science and Technology, Toyosawa 2200-2, Fukuroi-shi, 436 Shizuoka, Japan;(3) Department of Pharmacy, Hamamatsu University School of Medicine, Japan;(4) Department of Material Chemistry, Faculty of Science and Technology, Ryukoku University, Setaoue-cho Yokoya 1-5, Outsu-shi, 520-21 shiga, Japan |
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| Abstract: | Summary A fluorescent high-performance liquid chromatography (HPLC) analysis of folic acid (pteroylglutamic acid) and its derivatives using permanganate as a fluorogenic reagent has been developed. In the present work, the various analytical conditions in which the fluorogenic reaction with permanganate could be applied to the HPLC of folic acid in human serum, have been studied. The present method may be employed for the rapid clinical analysis of small amounts of folic acid ( 150 ppb), without interference from coexisting natural fluorophors; the precision of the present method for folic acid was 6.6% (n=6) (coefficient of variation).Dedicated to Professor Dr. Wilhelm Fresenius on the occasion of his 80th birthdayA part of this paper was presented at the 12th International Symposium on Microchemical Techniques, Cordoba (Spain), September 1992 |
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