Reaction-based genetically encoded fluorescent hydrogen sulfide sensors |
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Authors: | Chen Si Chen Zhi-jie Ren Wei Ai Hui-wang |
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Institution: | Department of Chemistry, University of California, 501 Big Springs Road, Riverside, California 92521, USA. |
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Abstract: | The detection of hydrogen sulfide (H(2)S), a toxic gas and an important biological signaling molecule, has been a long-time challenge. Here we report genetically encoded fluorescent protein (FP)-based probes that can selectively detect H(2)S. By expanding the genetic codes of E. coli and mammalian cells, FP chromophores were modified with the sulfide-reactive azide functional group. These structurally modified chromophores were selectively reduced by H(2)S, resulting in sensitive fluorescence enhancement detectable by spectroscopic and microscopic techniques. Exploration of a circularly permuted FP led to an improved sensor with faster responses, and the feasibility of using such a genetically encoded probe to monitor H(2)S in living mammalian cells has also been demonstrated. |
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