Analysis of fluorescence quenching of pyronin B and pyronin Y by molecular oxygen in aqueous solution |
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Authors: | Neslihan Çelebi |
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Institution: | Faculty of Arts and Sciences, Department of Chemistry, Atatürk University, 25240 Erzurum, Turkey |
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Abstract: | The fluorescence quenching of pyronin B and pyronin Y molecules by molecular oxygen in aqueous solution was studied by using steady-state and time-resolved fluorescence and UV-Vis absorption spectroscopy techniques. In order to understand the quenching mechanism, fluorescence decays, absorption and fluorescence spectra of the probes were recorded as a function of the oxygen concentration and temperature. The quenching was found to be appreciable and shows positive deviation in the Stern-Volmer representation obtained from the fluorescence intensity ratio. Fluorescence quenching constants (kq) were calculated from the τo/τ vs. Q] plots having linear correlation and compared with calculated diffusion-controlled rate constants (kdiff) values. Experimental results were in good agreement with the simultaneous dynamic and static quenching model. |
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Keywords: | Pyronin B and pyronin Y Dynamic and static quenching Molecular oxygen |
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